International Journal of Ecotoxicology and Ecobiology

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Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ

We studied on the ectomycorrhiza formation of Tricholoma matsutake on mature pine (Pinus densiflora) root using matsutake solid spawn in situ. To find out suitable medium for the solid spawn, the matsutake mycelium growths on different media were compared. Among them, GYP medium (glucose 20g, yeast extract 5g, peptone 5g, KH2PO4 1g, CaCl2 50mg and vitamin B1 50mg) and GWR medium (glucose 20g, wheat bran 50g, rice bran 50g, KH2PO4 1g, CaCl2 50mg and vitamin B1 50mg) showed good proliferation of matsutake mycelium. The GYP and GWR media were used to make charcoal and vermiculite-soil spawns. The ergosterol amounts of charcoal and vermiculite-soil spawns were 1.53 and 1.61 times higher than that of the natural shiro, respectively. In spring, the roots of about 30-year-old red pine were shortened with unsterilized scissors. About twelve months later, the regenerated root tips from shortened points were inoculated with charcoal and vermiculite-soil spawns, respectively. After four months by the inoculation with charcoal and vermiculite-soil spawns, 55% and 50% of experimental plots inoculated with charcoal and vermiculite-soil spawns revealed dichotomic root tips characteristic to ectomycorrhiza, respectively. The nested PCR amplification results confirmed that the dichotomous root tips were colonised by matsutake spawn. Our findings demonstrated that the ectomycorrhiza of T. matsutake could be formed on the mature pine root in situ by using matsutake solid spawns.

Tricholoma matsutake, Ectomycorrhiza, Solid Spawn, Inoculation, Pinus desiflora, Nested PCR

APA Style

Yong Chan Kim, Kol Nam, Myong Song Kim, Un Chol Kim, Un Hyok Kim, et al. (2023). Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ. International Journal of Ecotoxicology and Ecobiology, 8(3), 41-48.

ACS Style

Yong Chan Kim; Kol Nam; Myong Song Kim; Un Chol Kim; Un Hyok Kim, et al. Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ. Int. J. Ecotoxicol. Ecobiol. 2023, 8(3), 41-48. doi: 10.11648/j.ijee.20230803.13

AMA Style

Yong Chan Kim, Kol Nam, Myong Song Kim, Un Chol Kim, Un Hyok Kim, et al. Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ. Int J Ecotoxicol Ecobiol. 2023;8(3):41-48. doi: 10.11648/j.ijee.20230803.13

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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