Volume 5, Issue 4, December 2020, Page: 54-60
Influence of Mercury Exposure on Oxidative Stress Biomarkers in Longitudinal Muscle of Holothuria forskali
Imen Rabeh, Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Khaoula Telahigue, Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Tarek Hajji, Higher Institute of Biotechnology - Sidi Thabet, Biotechpole Sidi Thabet, Univ. Manouba, Ariana, Tunisia
Chaima Fouzai, Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Safa Bejaoui, Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Lassaad Chouba, National Institute of Marine Science and Technology (INSTM), La Goulette, Tunis, Tunisia
Mhamed El Cafsi, Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Nejla Soudani, Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Received: Nov. 20, 2020;       Accepted: Dec. 9, 2020;       Published: Dec. 16, 2020
DOI: 10.11648/j.ijee.20200504.13      View  69      Downloads  17
Abstract
Mercury (Hg) pollution is featuring as one of the major threat for marine ecosystem, biota and human health. Thereby, the objective of the current study was to evaluate the effect of environmentally relevant concentrations of mercury on oxidative stress, enzymatic and non-enzymatic antioxidant defenses and metallothionein levels in the longitudinal muscle of sea cucumber Holothuria forskali. With this purpose, sea cucumber were exposed to graded concentrations of mercury chloride HgCl2 (40, 80 and 160 µg L-1) for 96 h under controlled conditions. Our findings revealed that Hg burden in the longitudinal muscle tended to increase with increasing HgCl2 concentrations. The Hg exposure promoted muscular oxidative stress as evidenced by the increased levels of malondialdehyde (MDA), advanced oxidation protein product (AOPP) and metallothionein (MT) of treated animals compared with controls. Additionally, significant increases in the activities of the enzymatic (glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT)) and non-enzymatic (non-protein thiol (NPSH) and glutathione (GSH)) antioxidants were also observed in all treated groups. Overall, this study proved that mercury is able to produce deleterious effects even at the lowest environmentally-realistic concentration in the Holothuria forskali longitudinal muscle which may be considered as a target tissue of mercury accumulation in holothurian.
Keywords
Holothuria forskali, Mercuric Chloride (HgCl2), Longitudinal Muscle Hg Burden, Enzymatic and Non-enzymatic Antioxidant
To cite this article
Imen Rabeh, Khaoula Telahigue, Tarek Hajji, Chaima Fouzai, Safa Bejaoui, Lassaad Chouba, Mhamed El Cafsi, Nejla Soudani, Influence of Mercury Exposure on Oxidative Stress Biomarkers in Longitudinal Muscle of Holothuria forskali, International Journal of Ecotoxicology and Ecobiology. Vol. 5, No. 4, 2020, pp. 54-60. doi: 10.11648/j.ijee.20200504.13
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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