The anthropogenic activities culminating in environmental pollution usually lead to release of plethora of pollutants such as cadmium calls for concern. The effects of CdCl2 on the production of aspartate amino transferase (AST) in C. gariepinus and how they can be ameliorated through administration of vitamins were investigated. C. gariepinus fingerlings (whose initial weight ranged from 3-11g) were exposed to sub-lethal concentrations of Cd (00, 12mg/L, 16mg/L, 20mg/L and 24mg/L) with replicate in each case. In each case, 12mg/L of the vitamin was administered across all buds. Fresh concentrations of both toxicant and vitamins were administered every 72 hours for a period of 12 weeks every time the water medium was changed. The various treatments group include Cd only, CdVA, CdVC and CdVE. 3 samples of the fish were randomly selected and sacrificed from each aquarium tank every 2 weeks of the exposure period. The gills, kidneys and liver were excised from these specimens and homogenized in sodium phosphate buffer. From the results: in Cd only, the highest AST produced in the liver was 135.00±0.18nM/mg in T1. The highest AST produced in the kidneys of the fish was 145.00±0.18nM/mg in T3. The highest AST produced in the gill was 137.97±0.09nM/mg in T1. In CDVA samples, the highest AST produced in the liver was 132.19±0.18nM/mg in T4. The highest AST produced in the kidneys was 113.91±0.09nM/mg in T1. In the gills, the highest AST value was 120.94±0.36nM/mg in T1. In the samples exposed to CdVC, the highest AST produced in the liver was 128.44±0.36nM/mg in T1. The highest mean value of AST produced in the kidneys was 114.84±0.09nM/mg in T3. In the gills of the samples, the highest AST value was 125.16±0.27nM/mg in T2. In CdVE samples, the highest AST produced in the liver was 150.63±0.18nM/mg in T2. The kidneys’ highest AST value of 125.78±0.27nM/mg was recorded in T4. In the gills of the samples, the highest AST produced in the gills of the fish was 133.28±0.09nM/mg in T1. There were general high production levels of AST in all treatments with the highest values recorded in the liver of CdVA, CdVC and CdVE groups mostly in samples exposed to lower concentrations. The kidneys in the Cd only group however, produced the highest AST value. The high production values of AST in all treatments suggest that the enzyme is a good biomarker of oxidative stress elicited by the presence of the toxicant.
| Published in | International Journal of Ecotoxicology and Ecobiology (Volume 6, Issue 1) |
| DOI | 10.11648/j.ijee.20210601.15 |
| Page(s) | 19-28 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Clarias gariepinus, Aspartate Amino Transferase, Ameliorative Roles, Vitamin Supplements, Cd Treatment Groups
| [1] | Abdel-Warith, A. A., Younis, E. M., Al-Asgah, N. A., & Wahbi, O. M. (2011). Effect of zinc toxicity on liver histology of Nile tilapia, Oreochromis niloticus. Scientific Research Essential, 6 (17), 3760-3769. |
| [2] | Adewolu, M. A., Adeniji, C. A., & Adejobi, A. B. (2008). Feed utilization, growth and survival of Clarias gariepinus (Burchell, 1822) fingerlings cultured under different photoperiods. Aquaculture, 283, 64-67. |
| [3] | Ahmad, I., Maria, V. L., Oliveira, M., Serafim, A. Bebianno, M. J., Pacheco, M., & Santos, M. A. (2008). DNA damage and lipid peroxidation vs. protection responses in the gill of Dicentrarchus labrax L. from a contaminated coastal lagoon (Ria de Aveiro, Portugal). Science of the Total Environment, 406, 298-307. |
| [4] | Ahmed, N. F., Sadek, K. M., Soliman, M. K., Khalil, R. H., Khafaga, A. F., Ajarem, J. S., Maodaa, S. N., Allam, A. A. (2020). Moringa Oleifera Leaf Extract Repairs the Oxidative Misbalance following Sub-Chronic Exposure to Sodium Fluoride in Nile Tilapia Oreochromis niloticus. Animals, 10, 626. |
| [5] | Ali, S., Hussain, S., & Ulhaq, M. (2019). Renal toxicity of heavy metals (Cadmium and Mercury) and their amelioration with ascorbic acid in rabbits. Environmental Science and Pollution Research, 26, 3909-3920. |
| [6] | Arenas, J. J., Villafranca, M., Nieto-Guindo, E., ´Alvaro S. M., Moreno, S. M., Garrido, S., & Abil´es, J. (2017). Effects of cyclic parenteral nutrition on parenteral-associated liver dysfunction parameters. Nutrition Journal, 16, 1-8. |
| [7] | Athar, T., Waris, A. A. & Nisar, M. (2018). A review on toxicity and environmental implications of heavy metals. Emergent Life Sciences Research, 4 (2), 31-37. Doi: https://doi.org/10.31783/elsr.2018. |
| [8] | Ayyat, M. S., Ayyat, A. M., Naiel, M. A., Al-Sagheer, A. A. (2020). Reversal effects of some safe dietary supplements on lead contaminated diet induced impaired growth and associated parameters in Nile tilapia. Aquaculture, 515, 734580. |
| [9] | Bakare, A. A., Patel, S., Pandey, A. K., Bajpayee, M., & Dhawan, A. (2013). DNA and oxidative damage induced in somatic organs and tissues of mouse by municipal sludge leachate. Toxicology and Industrial Health, 28, 614–623. |
| [10] | Das, P. C., Ayyappan, S., Das, B. K., & Jena, J. K. (2004). Nitrite toxicity in Indian major carps: sublethal effect on selected enzymes in fingerlings of Catla catla, Labeo rohita, and Cirrhinus mrigala. Comparative Biochemistry and Physiology, 138, 3 – 10. |
| [11] | Ellakany, H., & Gaafar, H. (2002). Effects of combined aflatoxicosis and ochratoxicosis on immunological, biochemical and histopathological measurements in broilers. 6th Vet. Zag. Conference (7-9 Sept. 2002), Hurghada. |
| [12] | El-Said El-Boshy, M., Gadalla, H. A., & AbdEl-Hamied, F. M. (2014). Immunological, haematological and biochemical changes induced by short term exposure to cadmium in catfish (Clarias gariepinus). Journal of Coastal Life medicine, 2 (3), 175-180. Doi: 10.12980/JCLM.2.2014J57. |
| [13] | El-Shenawy, N. S., & Al-Ghamdi, O. A. (2014). Phenthoate induced oxidative stress in fresh isolated mice hepatocytes. Alleviation by ascorbic acid. Toxicology, Environment and Health Science, 6 (2), 67–80. |
| [14] | Ensibi, C., Perez-Lopez, M., Soler-Rodriguez, F., Miguez-Santiyan, M. P., Daly-Yahia, M. N., & Hernandez-Moreno, D. (2013). Effects of deltamethrin on biometric parameters and liver biomarkers in common carp (Cyprinus carpio L.). Environmental Toxicology and Pharmacology, 36, 384-391. |
| [15] | FAO (2003). Food Security: concepts and measurement. Rome: Food and Agriculture Organization of the United Nations. In FAO (Ed.), Trade Reforms and Food Security. pp. 25-34. |
| [16] | Feng, Y., Huang, X., Duan, Y., Fan, W., Duan, J., Wang, K., ……, & Yang, S. (2018). The Effects of Vitamin E and Metallothionein on the AntioxidantCapacities of Cadmium-Damaged Liver in Grass Carp, Ctenopharyngodon idellus. BioMed Research International, 1-8. https://doi.org/10.1155/2018/7935396. |
| [17] | Kaoud, H., Zaki, M., El-Dahshan, A. R., Abdelgayed, S. S. (2011). Ameliorating the toxic effects of cadmium-exposure in Nile Tiapia (Oreochromis niloticus) by using Lemna gibba L. Life Sciences, 8 (1), 185-195. |
| [18] | Karami, A., Christianus, A., Ishak, Z., Courtenay, S. C., Sayed, M. A., Noor, A. M., & Noor, S. H. (2010). Effect of triploidization on juvenile African catfish (Clarias gariepinus). Aquaculture International, 18, 851-858. |
| [19] | Mehana, E. E., Khafaga, A. F., Elblehi, S. S., AbdEl-Hack, M. E., Naiel, M. A. E., Bin-Jumah, M., SarahI. Othman, S. I., & Allam, A. A. (2020). Biomonitoring of Heavy Metal Pollution Using Acanthocephalans Parasite in Ecosystem: An Updated Overview. Animals, 10, 811, 1-15. doi: 10.3390/ani10050811. |
| [20] | Merciai, R., Guasch, H., Kumar, A., & Sabater, S. (2014). Trace metal concentration and fish size: variation among fish species in a Mediterranean river. Ecotoxicology and Environmental Safety, 107, 154–163. |
| [21] | Monteiro, D. A., Rantin, F. T., & Kalinin, A. L. (2010). Inorganic mercury exposure: toxicological effects, oxidative stress biomarkers and bioaccumulation in the tropical freshwater fish matrinx, Brycon amazonicus (Spix and Agassiz, 1829). Ecotoxicology, 19, 105-123. |
| [22] | Organization for Economic Cooperation and Development (2007). Maximum Acceptable Contaminants: guidance safety level. Fresh water fish (RPWS 1991). Pp. 12-28. |
| [23] | Pratt, T. C., Cullen, F. T., Sellers, C. S., Thomas, W. L., Madensen, T. D., Daigle, L. E., Fearn, N. E., & Gau, J. (2010). The empirical status of social learning theory: a meta-analysis. Justice Quarterly, 27: 6, 765-802. Doi: 10.1080/07418820903379610. |
| [24] | Reitman, S., & Frankel, S. (1957). Glutamic-Pyruvate transaminase assay by colorimetric method. American Journal of Clinical Pathology, 28- 56. |
| [25] | Saglam, D., Atli, G., Dogan, Z., Baysoy, E., Gurler, C., Eroglu, A., & Canli, M. (2014). Response of the antioxidant system of freshwater fish (Oreochromis niloticus) exposed to metals (Cd, Cu) in different hardness. Turkish Journal of Aquatic Science, 14, 43–52. |
| [26] | Shalaby, A. M. E. (2009). The opposing effects of ascorbic acid (Vit C) on ochratoxin toxicity in Nile Tilapia (Oreochromis niloticus). 6th International Symposium on Tilapia in Aquaculture, Phillipines, p150-157. |
| [27] | Shilpi, G., Shilpi, S., & Sunita, S. (2015). Tolerance Against Heavy Metal Toxicity In Cyanobacteria: Role Of Antioxidant Defense System. International Journal of Pharmacy and Pharmaceutical Sciences, (7) (2), 0975-1491. |
| [28] | Thakur, V., & Kanshere, R. R. (2014). Comparative Study on the Protective Role of Vitamin C and Vitamin E on Mercury Induced Toxicity in Heteropneusts fossilis. International Research Journal of Science & Engineering, 2 (2), 37-43. |
| [29] | Van Der Oost, R., Beyer, J., & Vermeulen, N. P. E. (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: A review. Environmental Toxicology and Pharmacology, 13, 57-149. |
| [30] | Yancheva, V., Stoyanova, S., Velcheva, I., Petrova, S., & Georgieva, E. (2014). Metal bioaccumulation in common carp and rudd from the Topolnitsa reservoir, Bulgaria. Archives of industrial hygiene and toxicology, 65 (1), 1-10. DOI: 10.2478/10004-1254-65-2014-2451. |
| [31] | Yolanda, M., & Maria, L. I. (2012). Use of antioxidants for the treatment of cognitive and behavioural disorders in individuals with fragile X syndrome. WO2012080554. |
| [32] | Zikic, R. V., Stajn, S., Pavlovic, Z., Ognjanovic, B. I., & Saicic, Z. S. (2001). Activities of superoxide dismutase and catalase in erythrocyte and plasma transaminases of goldfish (Carassius auratus gibelio Bloch.) exposed to cadmium. Physiological Research, 50, 105–111. |
APA Style
Samuel Patrick Ozovehe, Arimoro Francis Ofurum, Ayanwale Adesola Victoria, Mohammad Hadiza Lami. (2021). Evaluation of the Ameliorative Roles of Vitamins A, C and E on Aspartate Amino Transferase in Clarias gariepinus (Burchell, 1822) Fingerlings Exposed to Camium Chloride. International Journal of Ecotoxicology and Ecobiology, 6(1), 19-28. https://doi.org/10.11648/j.ijee.20210601.15
ACS Style
Samuel Patrick Ozovehe; Arimoro Francis Ofurum; Ayanwale Adesola Victoria; Mohammad Hadiza Lami. Evaluation of the Ameliorative Roles of Vitamins A, C and E on Aspartate Amino Transferase in Clarias gariepinus (Burchell, 1822) Fingerlings Exposed to Camium Chloride. Int. J. Ecotoxicol. Ecobiol. 2021, 6(1), 19-28. doi: 10.11648/j.ijee.20210601.15
AMA Style
Samuel Patrick Ozovehe, Arimoro Francis Ofurum, Ayanwale Adesola Victoria, Mohammad Hadiza Lami. Evaluation of the Ameliorative Roles of Vitamins A, C and E on Aspartate Amino Transferase in Clarias gariepinus (Burchell, 1822) Fingerlings Exposed to Camium Chloride. Int J Ecotoxicol Ecobiol. 2021;6(1):19-28. doi: 10.11648/j.ijee.20210601.15
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Download@article{10.11648/j.ijee.20210601.15,
author = {Samuel Patrick Ozovehe and Arimoro Francis Ofurum and Ayanwale Adesola Victoria and Mohammad Hadiza Lami},
title = {Evaluation of the Ameliorative Roles of Vitamins A, C and E on Aspartate Amino Transferase in Clarias gariepinus (Burchell, 1822) Fingerlings Exposed to Camium Chloride},
journal = {International Journal of Ecotoxicology and Ecobiology},
volume = {6},
number = {1},
pages = {19-28},
doi = {10.11648/j.ijee.20210601.15},
url = {https://doi.org/10.11648/j.ijee.20210601.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijee.20210601.15},
abstract = {The anthropogenic activities culminating in environmental pollution usually lead to release of plethora of pollutants such as cadmium calls for concern. The effects of CdCl2 on the production of aspartate amino transferase (AST) in C. gariepinus and how they can be ameliorated through administration of vitamins were investigated. C. gariepinus fingerlings (whose initial weight ranged from 3-11g) were exposed to sub-lethal concentrations of Cd (00, 12mg/L, 16mg/L, 20mg/L and 24mg/L) with replicate in each case. In each case, 12mg/L of the vitamin was administered across all buds. Fresh concentrations of both toxicant and vitamins were administered every 72 hours for a period of 12 weeks every time the water medium was changed. The various treatments group include Cd only, CdVA, CdVC and CdVE. 3 samples of the fish were randomly selected and sacrificed from each aquarium tank every 2 weeks of the exposure period. The gills, kidneys and liver were excised from these specimens and homogenized in sodium phosphate buffer. From the results: in Cd only, the highest AST produced in the liver was 135.00±0.18nM/mg in T1. The highest AST produced in the kidneys of the fish was 145.00±0.18nM/mg in T3. The highest AST produced in the gill was 137.97±0.09nM/mg in T1. In CDVA samples, the highest AST produced in the liver was 132.19±0.18nM/mg in T4. The highest AST produced in the kidneys was 113.91±0.09nM/mg in T1. In the gills, the highest AST value was 120.94±0.36nM/mg in T1. In the samples exposed to CdVC, the highest AST produced in the liver was 128.44±0.36nM/mg in T1. The highest mean value of AST produced in the kidneys was 114.84±0.09nM/mg in T3. In the gills of the samples, the highest AST value was 125.16±0.27nM/mg in T2. In CdVE samples, the highest AST produced in the liver was 150.63±0.18nM/mg in T2. The kidneys’ highest AST value of 125.78±0.27nM/mg was recorded in T4. In the gills of the samples, the highest AST produced in the gills of the fish was 133.28±0.09nM/mg in T1. There were general high production levels of AST in all treatments with the highest values recorded in the liver of CdVA, CdVC and CdVE groups mostly in samples exposed to lower concentrations. The kidneys in the Cd only group however, produced the highest AST value. The high production values of AST in all treatments suggest that the enzyme is a good biomarker of oxidative stress elicited by the presence of the toxicant.},
year = {2021}
}
TY - JOUR T1 - Evaluation of the Ameliorative Roles of Vitamins A, C and E on Aspartate Amino Transferase in Clarias gariepinus (Burchell, 1822) Fingerlings Exposed to Camium Chloride AU - Samuel Patrick Ozovehe AU - Arimoro Francis Ofurum AU - Ayanwale Adesola Victoria AU - Mohammad Hadiza Lami Y1 - 2021/03/30 PY - 2021 N1 - https://doi.org/10.11648/j.ijee.20210601.15 DO - 10.11648/j.ijee.20210601.15 T2 - International Journal of Ecotoxicology and Ecobiology JF - International Journal of Ecotoxicology and Ecobiology JO - International Journal of Ecotoxicology and Ecobiology SP - 19 EP - 28 PB - Science Publishing Group SN - 2575-1735 UR - https://doi.org/10.11648/j.ijee.20210601.15 AB - The anthropogenic activities culminating in environmental pollution usually lead to release of plethora of pollutants such as cadmium calls for concern. The effects of CdCl2 on the production of aspartate amino transferase (AST) in C. gariepinus and how they can be ameliorated through administration of vitamins were investigated. C. gariepinus fingerlings (whose initial weight ranged from 3-11g) were exposed to sub-lethal concentrations of Cd (00, 12mg/L, 16mg/L, 20mg/L and 24mg/L) with replicate in each case. In each case, 12mg/L of the vitamin was administered across all buds. Fresh concentrations of both toxicant and vitamins were administered every 72 hours for a period of 12 weeks every time the water medium was changed. The various treatments group include Cd only, CdVA, CdVC and CdVE. 3 samples of the fish were randomly selected and sacrificed from each aquarium tank every 2 weeks of the exposure period. The gills, kidneys and liver were excised from these specimens and homogenized in sodium phosphate buffer. From the results: in Cd only, the highest AST produced in the liver was 135.00±0.18nM/mg in T1. The highest AST produced in the kidneys of the fish was 145.00±0.18nM/mg in T3. The highest AST produced in the gill was 137.97±0.09nM/mg in T1. In CDVA samples, the highest AST produced in the liver was 132.19±0.18nM/mg in T4. The highest AST produced in the kidneys was 113.91±0.09nM/mg in T1. In the gills, the highest AST value was 120.94±0.36nM/mg in T1. In the samples exposed to CdVC, the highest AST produced in the liver was 128.44±0.36nM/mg in T1. The highest mean value of AST produced in the kidneys was 114.84±0.09nM/mg in T3. In the gills of the samples, the highest AST value was 125.16±0.27nM/mg in T2. In CdVE samples, the highest AST produced in the liver was 150.63±0.18nM/mg in T2. The kidneys’ highest AST value of 125.78±0.27nM/mg was recorded in T4. In the gills of the samples, the highest AST produced in the gills of the fish was 133.28±0.09nM/mg in T1. There were general high production levels of AST in all treatments with the highest values recorded in the liver of CdVA, CdVC and CdVE groups mostly in samples exposed to lower concentrations. The kidneys in the Cd only group however, produced the highest AST value. The high production values of AST in all treatments suggest that the enzyme is a good biomarker of oxidative stress elicited by the presence of the toxicant. VL - 6 IS - 1 ER -
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